<?xml version="1.0" encoding="UTF-8" ?>
<oai_dc:dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
<dc:title>Characterization of Mycoviruses in Armillaria ostoyae and A. cepistipes in the Czech Republic</dc:title>
<dc:creator>Walterová, Lucie</dc:creator>
<dc:creator>Botella, Leticia</dc:creator>
<dc:creator>Hejna, Ondřej</dc:creator>
<dc:creator>de la Peña, Marcos</dc:creator>
<dc:creator>Tonka, Tomáš</dc:creator>
<dc:creator>Čurn, Vladislav</dc:creator>
<dc:description>Members of the genus <i>Armillaria</i> are widespread forest pathogens against which effective protection has not yet been developed. Due to their longevity and the creation of large-scale cloning of <i>Armillaria</i> individuals, the use of mycoviruses as biocontrol agents (BCAs) against these pathogens could be an effective alternative. This work describes the detection and characterization of viruses in <i>Armillaria</i> spp. collected in the Czech Republic through the application of stranded total RNA sequencing. A total of five single-stranded RNA viruses were detected in <i>Armillaria ostoyae</i> and <i>A. cepistipes</i>, including viruses of the family <i>Tymoviridae</i> and four viruses belonging to the recently described “ambivirus” group with a circular ambisense genome arrangement. Both hammerhead (HHRz) and hairpin (HpRz) ribozymes were detected in all the ambiviricot sequences. <i>Armillaria</i> viruses were compared through phylogenetic analysis and confirmed their specific host by direct RT-PCR. One virus appears to infect both <i>Armillaria</i> species, suggesting the occurrence of interspecies transmission in nature.</dc:description>
<dc:date>2024-05-04T09:49:47Z</dc:date>
<dc:date>2024-05-04T09:49:47Z</dc:date>
<dc:date>2024-04-15</dc:date>
<dc:date>2024-05-04T09:49:47Z</dc:date>
<dc:type>artículo</dc:type>
<dc:identifier>doi: 10.3390/v16040610</dc:identifier>
<dc:identifier>Viruses 16 (4): 610 (2024)</dc:identifier>
<dc:identifier>http://hdl.handle.net/10261/355809</dc:identifier>
<dc:rights>open</dc:rights>
<dc:publisher>Multidisciplinary Digital Publishing Institute</dc:publisher>
</oai_dc:dc>